Biosensors are widely used to manage disease or track food allergens. They make use of enzymes that specifically detect a substance in a sample and convert it into a measurable signal. DirectSens has over ten years experience with a specific class of enzymes, which is capable of direct electron transfer. This feature enables a 3rd generation sensing mechanism, which is independent of mediators or oxygen, thus simplifies the architecture and increases performance. Sensors based on this enzyme technology are highly accurate, precise and robust.
In first-generation biosensors, the electrons are transferred to molecular oxygen and the resulting decrease in the oxygen concentration and/or the produced hydrogen peroxide is measured.
Second-generation biosensors use artificial, partially toxic mediators or nanomaterials to transport the electrons to the electrode.
In third-generation biosensors, the electrons are transferred directly from the enzyme to the electrode without any intermediate stages or use of nanoparticles.
3 rd Generation Glucose Sensing Enzyme
DirectSens is providing an glucose specific enzyme engineered towards the use in a Continuous Glucose Monitoring System (CGMS) that reliably monitors the glucose levels of Diabetes patients.
Glucose Sensor Reference Design
DirectSens provides a Reference Design of our Glucose Sensing technology. Enzymes, materials and procedures to realize prototypes are provided to aid validation of our technology in your laboratory.
Oxygen independency
Calibration in presence and absence of oxygen: Each calibration represents three independent sensors measured in the presence and absence of oxygen. The red curve describes the current response of sensors submersed in N2-purged PBS buffer, while the black values where recorded without purging. The experimental data show the independency of the sensing mechanism of oxygen.
Interference Studies
Each substance was measured in the presence of 5 mM (90 mg dL-1) glucose at concentrations recommended by the CLSI POCT05-A guideline. The boxplot represents data of 5 repetitions. None of the tested substances altered the sensor readout above 10%. Note, no selective membrane was applied.
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